|
KMID : 0380020050200010026
|
|
Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 1 p.26 ~ p.33
|
|
|
Effects of Carbon Substrates on Exopolysaccharide Production by Enterobacter sp.
|
|
Lee Joo-Ha
Lee Shin-Young
|
|
|
Abstract
|
|
|
|
The effects of carbon sources for exopolysaccharide production during batch cultivation of an Enterobacter sp. isolated from the composter were investigated. The highest amount of exopolysaccharide was obtained when lactose was used as carbon source. Lactose in medium was converted into glucose and galactose. Glucose was metabolized fast and was completely consumed, but about of lactose was accumulated as galactose. On the other hand, enzyme activity was about unit with the increase of lactose concentration. Thus, it was considered that the exopolysaccharide might be produced in the course of that lactose was hydrolyzed into glucose and galactose by with respect to that enzyme activity on lactose hydrolysis was accorded to the exopolysaccharide production. When glucose and galactose were added to lactose medium, respectively, it could be considered that glucose was as a repressor and galactose was as a inducer for synthesis even though the mechanisms were not elucidated. The increase of lactose concentration was almost ineffective to the specific growth rate but showed the difference in the biomass content. The higher carbon source concentration, the more residual sugar remained. It was assumed that the optimum lactose concentration for exopolysaccharide production was On the other hand, it was considered that the nitrogen acted as growth limiting nutrients to the cell growth. In the cases of 30 and 70 g/L of the fixed carbon concentrations, the increase of the nitrogen sources concentration caused a remarkable increase within the range of and of the specific growth rate, respectively, while there was no significant difference in biomass.
|
|
|
KEYWORD
|
|
|
Enterobcater sp., exopolysaccharide production, carbon substrates
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|